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Infrastructure and equipments

Plateformes
Published on 27 April 2022
  • Liquid handling automation equipped with a 96-well head + 8 individual channels (Microlab Star, Hamilton), placed under type II containment (BigNeat).
  • High content imaging automated microscope (epifluorescence & spinning-disk pseudo-confocal imaging, 4 colors + brightfield. Operetta, Perkin-Elmer). 
  • Multimode microplate reader (fluorescence/luminescence/spectrophotometer) equipped with 2 injectors for flash measurements (Mithras LB940, Berthold).
  • High-speed reagent dispenser in microplate format (Multidrop combi, Thermo).
  • Plate washer (Elx-405, Biotek).
  • Particles counter (Z2, Beckman-Coulter).
  • Libraries of biological effectors:

     - Human, genome-wide siRNA library (18000 target genes, pools of 4 siRNAs/gene, « On-Target Plus » library, Thermo).

       - Human, miRnome-wide miRNA knock-down library (994 LNAs, 1 LNA/miRNA, miRBase V19, Exiqon).

    - Human, miRnome-wide miRNA overexpression library (2050 miRNA mimics, 1 mimic/miRNA, miRBase V19, Thermo).

    - Human, miRnome-wide miRNA knock-down library (2050 miRNA antisenses, 1 hairpin/miRNA, miRBase V19, Thermo).

      - Repurposable drug library (1520 small molecules, 98% « FDA-approved », Prestwick Chemicals).