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Group leader : Frédéric Ducongé
EMAIL : Frédéric Ducongé
For several years, we have been developing innovative methods for the selection of aptamers that have led to several patents. Our aim is to use these aptamers to study the mechanisms involved in neurodegenerative diseases and to develop new therapeutic and diagnostic approaches.
Modeled structure of an aptamer
Aptamers are high affinity ligands made up of three-dimensional structures of nucleic acids (RNA, DNA or modified chemistry). They can be selected by in vitro molecular evolution methods against many molecular targets (e. g. proteins, peptides or small chemical molecules such as metabolites or neurotransmitters). Aptamers have several advantages over antibodies. For example, they can be chemically produced and are very stable over a wide range of pH and temperature. They can thus be easily combined with many compounds to create new specific molecular recognition probes.
Our research activity concerns:
1- the selection of aptamers that can specifically recognize different types of protein aggregates involved in neurodegenerative diseases.
2- the selection of aptamers that can be used for in vivo imaging of gene expression.
3- the selection of aptamers that can inhibit proteins involved in neuronal death.
Laboratory Members Associated with Projects
Nucleic acid aptamers for neurodegenerative diseasesBouvier-Müller, A. and Ducongé, F. Biochimie 2017 j.biochi.2017.10.026.
DNA aptamer affinity ligands for highly selective purification of human plasma-related proteins from multiple sourcesForier, C., Boschetti, E., Ouhammouch, M., Cibiel, A., Duconge, F., Nogre, M., Tellier, M., Bataille, D., Bihoreau, N., Santambien, P. et al. J Chromatogr 2017 A, 1489, 39-50.
Applications of High-Throughput Sequencing for In Vitro Selection and Characterization of AptamersNguyen Quang, N., Perret, G. and Ducongé, F. Pharmaceuticals 2016, 9, 76.
How to Measure the Affinity of Aptamers for Membrane Proteins Expressed on the Surface of Living Adherent CellsQuang, N.N., Pestourie, C., Cibiel, A. and Duconge, F. Methods 2016, 97, 35-43.
In Vitro and In Vivo Imaging of Fluorescent AptamersTheodorou, I., Quang, N.N., Gombert, K., Theze, B., Lelandais, B. and Duconge, F. Methods Mol Bio 2016, 1380, 135-150.1.
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.