The François Jacob Institute of Biology brings together five departments and three services
The last two years in scientific news
A defect in the repair of oxidative DNA damages can result in the accumulation of mutations and even induce cell death and has been involved in many human pathologies such as cancer or neurodegenerative diseases. Our laboratory is interested in the repair of oxidative DNA damages by the Base Excision Repair pathway, that is essential to ensure the stability of both nuclear and mitochondrial genomes. One of the most abundant base lesions induced by oxidative stress is the 8-oxoguanine (8-oxoG) that is excised by the DNA glycosylase OGG1.
Tumoral plasticity, characterized by the existence of cancer stem cells and by the epithelial mesenchymal transition (EMT), is at the origin of intra-tumoral heterogeneity and plays a major role in the resistance to radio or chemotherapy. We are interested in understanding how the modification of the redox state is linked to this process and affects acquired cellular resistance. We are particularly interested in the role of the DNA glycosylase OGG1, in both cellular plasticity and resistance to the treatments.
Image of structure illumination microscopy showing a cell expressing the DNA repair protein OGG1 (in green) in both the nucleus and the mitochondria. Mitochondrial network is visualized using Mitotracker Red .
By using a combination of molecular biology, genomics, super-resolution microscopy and cellular biology techniques applied to both in vitro and in vivo models, our projects focus on the understanding and modulation of the cellular responses induced by exposure to oxidizing agents or ionizing radiation. Chemical molecules and metallic nanoparticles modulating the redox state of the cells and inducing DNA instability are under study for their potential as new therapies for the treatment of cancer or neurodegenerative disorders.
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.