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Immunohistochemistry and anatomopathology


The immunohistochemistry and anatomopathology platforms are equipped according to highest technical standards with biosecurity levels A1 and A3. It provides macroscopic, histopathologic and molecular analysis on rodents and non-human primates.
Published on 3 July 2018
Immunohistochimie et anatomopathologie

These platforms are under the responsibility of engineers specialized in the nervous central system field and of a doctor of veterinary medicine, diplomate of European College of Veterinary Pathologists.

These platforms provide expertise and consulting for academic and industrial research partners on a collaborative collaborative or service basis. This encloses the realization of “classical” evaluations in anatomic pathology, including macroscopic and histopathologic analyses, as well as the development and evaluation of IHC, IF and ISH protocols. Image analysis, morphometry and automatic cell count complete the expertise provided by the platform.

Immunohistochemistry

This platform is dedicated of histological analysis in rodents, non-human primates and human brain tissues. The platform benefit of a large laboratory space provided with many high-tech and safety equipments ranging from tissue sampling to microscopy analysis.

ACTIVITIES

The histology platform team is specialized in nervous system histology and performs several types of histological techniques and developments (histological staining, enzymatic labeling, immunochemistry with chromogens or fluorescence, autoradiography, in situ hybridization) and carries out qualitative and quantitative analysis. The team also provides training for people who want to learn these techniques.

EQUIPMENTS


Sample collection :

  • Macroscopy tables, located in biosafety level 2 and 3 areas, dedicated to tissue sampling and tissue fixation for rodents and primates.

Tissue sectioning :

  • Cryostats (Leica CM 3050S)
  • Freezing microtomes (Leica SM 2400)
  • Vibratome (Leica VT 1000)

Automatic labeling:

  • Discovery XT Ventana for reproducible immunochemistry and in situ hybridization.

Analysis:

  • Light and fluorescence motorized microscopes (LEICA DM6000, Zeiss AxioPlan Imaging)
  • Light and fluorescence macroscope (Leica Z16 APO)
  • Digital slides scanner (Zeiss Axioscan Z1)
  • Software for automatic acquisition of section (mosaic pattern) and automatic detection of objects (Explora Nova).
  • Software for unbiased stereology (Explora Nova).


​Tau expression after injection of a viral vector into a rodent hippocampus



Amyloïde plaques (green) surrounded by astrocytes (red) (nucleus, blue)


Anatomopathology

Studies are proceeded according to projects : 

1. Evaluation and characterization of animal models (in non-human primates and rodents) of neurodegenerative diseases.

2. Evaluation of efficacy, biodistribution and toxicity of experimental therapeutics or viral vectors.


Hematoxylin and eosin stain of diseased brain (left) and lung (right) and May-Grünwald Giemsa stain of amnion fluid (middle)
 
lev-2-demi.jpg  
Immunohistochemistry (Ventana DISCOVERY XT; left) and corresponding morphometric analysis (NIS-Elements Br; right) of stained cells (red) and non-stained cells (green) of a pancreas section