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Laboratory of Experimental Oncology - LCE

Published on 24 April 2019
Research Programs of the Laboratory of Experimental Cancerology (LCE) are dedicated to the analysis of the effects and the risk associated with radiation exposure at low doses.
Sylvie Chevillard
Team Leader
Phone : 33 (0) 1 45 54 88 89
sylvie.chevillard@cea.fr​​


A​nalysis of the late effect of radiation exposure: molecular characterization of radiation-induced thyroid tumors ​

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Our laboratory identified two transcriptomic signatures discriminating sporadic and radiation-induced thyroid tumors. These signatures are robust enough to predict the etiology of thyroid tumors (New and larger independent series of tumors being currently analyzed to confirm this result). Moreover, these first studies highly suggested that post-radiotherapy (thyroid dose of 20-40Gy) and post-Chernobyl tumors (thyroid dose of a magnitude of a few dozen mGy to a few Gy) display a common core of molecular specificity, which is independent of the mode of exposure (external exposure, Iodine131 contamination, respectively) and the dose, especially, five genes were found in both signatures.









Research of radioresistance biomarkers in human carcinomas​
Cancer stem cells (CSC) play a key role in tumor resistance, including radiation resistance, and tumor metastasis. We have previously attributed a role of marker/actor to CD24 (CSC marker) in radiation resistance of breast cancer cells. We are currently studying its role in different carcinomas. This work, initiated in human cell lines, will be continued using tumor samples to evaluate CD24 expression as predictive/prognostic marker in clinical research.

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Flow cytometry characterization of human immortalized epithelial mammary cells upon treatment. Parental cells display essentially a CD24+/CD44low labelling, and treatment led to the appearance of a CD24-/CD44+ cell population (CSC population). CD24+/CD44low cells were morphologically cuboidal-like epithelial cells, and CD24-/CD44+ cells were fibroblast-like, mesenchymal cells.
 

 
Team working on the new molecular biomarkers of radioresistance/sensitivity, has identified new form of phosphoS27-S33-Ku70 in progressive CLL. Current studies explore the role (linked to DNA repair or not) of this protein in vitro (cancer cell lines, approach using shRNA vectors) and in vivo in generated mouse Knock-In model expressing human epitope of Ku70. The goal interest is a deciphering the causes of pathological deregulation of this protein in cancer.LCE-Photo 2b.png
Phospho​-S27-Ku70 Immunofluorescence 15min postirradiation (2Gy)​​

This research has applications in the treatment of certain cancers and radiation protection. We interact with the nuclear industry (EDF and AREVA).​