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Organic Chemistry and Biocatalysis Laboratory (LCOB)
A. Zaparucha, V. de Berardinis, C. Vergne-Vaxelaire "Genome mining for enzyme discovery", in "Modern Biocatalysis: Advances in Synthetic Biological Systems", editors: Gavin Williams and Mélanie Hall, the Royal Society of Chemistry, 2018, ISBN 978-1-78262-726-5.
a-ketoglutarate dependent dioxygenases (a-KetoAcid Oxygenases, aKAO).
Hydrolysis of nitriles to the corresponding carboxylic acids most frequently necessitates strong conditions, acidic or basic, which may give rise to undesirable byproducts. Enzymatic hydrolysis is an interesting alternative and spares fragile functional groups. Nitrilases are promiscuous enzymes; some of them are active on structurally very diverse substrates.
During a large-scale study, we identified 125 new nitrilases and determined their substrate profiles. We are particularly interested in enzymes able to convert
a-amino acids thus providing the scope for access to non-natural amino acids.
Enzymatic reaction cascades.
Various hydroxylated polyamines were synthesized from L-lysine by sequencing 1 or 2 stages of regiodivergent hydroxylation with decarboxylation. The synthesis, which is very short, enables access to chiral platform molecules.
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.